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pbluescript ii sk (+) plasmid vector  (Thermo Fisher)


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    Structured Review

    Thermo Fisher pbluescript ii sk (+) plasmid vector
    Pbluescript Ii Sk (+) Plasmid Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+ii+sk+(+)+plasmid+vector/pmc04115231-48-5-11
    Average 90 stars, based on 1 article reviews
    pbluescript ii sk (+) plasmid vector - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Autistic-like behaviours and hyperactivity in mice lacking ProSAP1/Shank2.
    Article Snippet: Michael J. Schmeisser*, Elodie Ey*, Stephanie Wegener*, Juergen Bockmann, A. Vanessa Stempel, Angelika Kuebler, Anna-Lena Janssen, Patrick T. Udvardi, Ehab Shiban, Christina Spilker, Detlef Balschun, Boris V. Skryabin, Susanne tom Dieck, Karl-Heinz Smalla, Dirk Montag, Claire S. Leblond, Philippe Faure, Nicolas Torquet, Anne-Marie Le Sourd, Roberto Toro, Andreas M. Grabrucker, Sarah A. Shoichet, Dietmar Schmitz, Michael R. Kreutz, Thomas Bourgeron, Eckart D. Gundelfinger & Tobias M. Boeckers

    Article Title: Alteration of Antiviral Signalling by Single Nucleotide Polymorphisms (SNPs) of Mitochondrial Antiviral Signalling Protein (MAVS)
    Article Snippet: HeLa cells (JCRB Cell Bank, Osaka, Japan) and stably MAVS-silenced 293-flp cells (293-flp MAVS KD cells, generated in our previous study [ ]) were maintained in a 5% CO 2 atmosphere at 37°C in Dulbecco’s modified Eagle’s medium (DMEM) (Sigma-Aldrich, St. Louis, MO) supplemented with 10% fetal bovine serum (FBS) (Perbio Science, Switzerland) and antibiotics (Invitrogen, Carlsbad, CA). .. To obtain the MAVS-wild type (WT) cloning vectors, the SalI-NotI site of the cDNA in pCMV-Myc-MAVS (generated in our previous study [ ]) was transferred into the cloning vector pBlueScript II SK(+) (Fermentas, Canada). .. MAVS SNP-cDNAs were generated by site-directed mutagenesis using the MAVS-WT-encoding vector pBlueScript II SK(+) as the template as well as the primer pairs shown in .

    Clone Assay:

    Article Title: Autistic-like behaviours and hyperactivity in mice lacking ProSAP1/Shank2.
    Article Snippet: Michael J. Schmeisser*, Elodie Ey*, Stephanie Wegener*, Juergen Bockmann, A. Vanessa Stempel, Angelika Kuebler, Anna-Lena Janssen, Patrick T. Udvardi, Ehab Shiban, Christina Spilker, Detlef Balschun, Boris V. Skryabin, Susanne tom Dieck, Karl-Heinz Smalla, Dirk Montag, Claire S. Leblond, Philippe Faure, Nicolas Torquet, Anne-Marie Le Sourd, Roberto Toro, Andreas M. Grabrucker, Sarah A. Shoichet, Dietmar Schmitz, Michael R. Kreutz, Thomas Bourgeron, Eckart D. Gundelfinger & Tobias M. Boeckers

    Sequencing:

    Article Title: Autistic-like behaviours and hyperactivity in mice lacking ProSAP1/Shank2.
    Article Snippet: Michael J. Schmeisser*, Elodie Ey*, Stephanie Wegener*, Juergen Bockmann, A. Vanessa Stempel, Angelika Kuebler, Anna-Lena Janssen, Patrick T. Udvardi, Ehab Shiban, Christina Spilker, Detlef Balschun, Boris V. Skryabin, Susanne tom Dieck, Karl-Heinz Smalla, Dirk Montag, Claire S. Leblond, Philippe Faure, Nicolas Torquet, Anne-Marie Le Sourd, Roberto Toro, Andreas M. Grabrucker, Sarah A. Shoichet, Dietmar Schmitz, Michael R. Kreutz, Thomas Bourgeron, Eckart D. Gundelfinger & Tobias M. Boeckers

    other:

    Article Title: Construction of a bovine enterovirus-based vector expressing a foot-and-mouth disease virus epitope.
    Article Snippet: A recombinant infectious bovine enterovirus (BEV) vector was constructed to express a foot-and-mouth disease virus (FMDV) capsid protein (VP1) epitope.. Sequences encoding the VP1 epitope (amino acid residues 141–160) of FMDV (vaccine strain O1/Manisa/Turkey/69) were inserted into pBLUBEV at the VP1/2A junction.. The growth characteristics of the parental virus and viruses derived from recombinant plasmids (pBLUBEV, pBLUBEV-Manisa-epi) were determined by plaque assay and one-step growth curve

    Article Title: Identification of drought-inducible regulatory factors in Lablab purpureus by a comparative genomic approach
    Article Snippet: Physiological indices of two lines of hyacinth bean (Lablab purpureus (L.) Sweet), MEIDOU 2012 and NANHUI 23, were compared during a progressive course of water-withholding treatments.. MEIDOU 2012 was shown to be a superior drought-tolerant genotype.. A suppressive subtractive hybridisation library was then constructed by using drought-stressed MEIDOU 2012 roots and sequenced to screen for transcripts with differential abundance variations.

    Article Title: Development of microsatellites from Cornus mas L. (Cornaceae) and characterization of genetic diversity of cornelian cherries from China, central Europe, and the United States
    Article Snippet: Cornelian cherry (Cornus mas L.) is indigenous to central and southeastern Europe and is an ecologically and economically important shrub or small tree.. The aim of this study was to develop molecular tools for assessing genetic diversity and provide unique molecular identification of C. mas samples from central Europe and United States.. A microsatellite-enriched library was used to develop nine polymorphic microsatellite loci.

    Article Title: Isolation and characterization of microsatellite loci for Cornus sanguinea (Cornaceae)
    Article Snippet: Microsatellite-enriched fragments were ligated to the pBluescript II SK (+) vector (Fermentas, Glen Burnie, Maryland, USA) and transformed into Escherichia coli TOP10 cells (Invitrogen, Carlsbad, California, USA).

    Article Title: Identification of Key Drought Stress-Related Genes in the Hyacinth Bean
    Article Snippet: Subtracted cDNAs were ligated into a pBluescript II SK(−) vector and transformed into E. coli strain DH10B (Invitrogen, Carlsbad, CA, USA).

    Article Title: Construction of a pIX-modified Adenovirus Vector Able to Effectively Bind to Nanoantibodies for Targeting
    Article Snippet: Plasmid vectors We used the pBluescript II SK (+) plasmid vector (Fermentas MBI, Lithuania); pGEM-T-Easy plasmid system (Promega, USA); pShuttle-CMV-EGFP shuttle vector containing the cytomegalovirus (CMV) promoter, enhanced green fluorescent protein (EGFP) reporter gene and Ad5 genomic fragments; and pAdEasy-1 plasmid (Stratagene, USA).

    Cloning:

    Article Title: Alteration of Antiviral Signalling by Single Nucleotide Polymorphisms (SNPs) of Mitochondrial Antiviral Signalling Protein (MAVS)
    Article Snippet: HeLa cells (JCRB Cell Bank, Osaka, Japan) and stably MAVS-silenced 293-flp cells (293-flp MAVS KD cells, generated in our previous study [ ]) were maintained in a 5% CO 2 atmosphere at 37°C in Dulbecco’s modified Eagle’s medium (DMEM) (Sigma-Aldrich, St. Louis, MO) supplemented with 10% fetal bovine serum (FBS) (Perbio Science, Switzerland) and antibiotics (Invitrogen, Carlsbad, CA). .. To obtain the MAVS-wild type (WT) cloning vectors, the SalI-NotI site of the cDNA in pCMV-Myc-MAVS (generated in our previous study [ ]) was transferred into the cloning vector pBlueScript II SK(+) (Fermentas, Canada). .. MAVS SNP-cDNAs were generated by site-directed mutagenesis using the MAVS-WT-encoding vector pBlueScript II SK(+) as the template as well as the primer pairs shown in .

    Generated:

    Article Title: Alteration of Antiviral Signalling by Single Nucleotide Polymorphisms (SNPs) of Mitochondrial Antiviral Signalling Protein (MAVS)
    Article Snippet: HeLa cells (JCRB Cell Bank, Osaka, Japan) and stably MAVS-silenced 293-flp cells (293-flp MAVS KD cells, generated in our previous study [ ]) were maintained in a 5% CO 2 atmosphere at 37°C in Dulbecco’s modified Eagle’s medium (DMEM) (Sigma-Aldrich, St. Louis, MO) supplemented with 10% fetal bovine serum (FBS) (Perbio Science, Switzerland) and antibiotics (Invitrogen, Carlsbad, CA). .. To obtain the MAVS-wild type (WT) cloning vectors, the SalI-NotI site of the cDNA in pCMV-Myc-MAVS (generated in our previous study [ ]) was transferred into the cloning vector pBlueScript II SK(+) (Fermentas, Canada). .. MAVS SNP-cDNAs were generated by site-directed mutagenesis using the MAVS-WT-encoding vector pBlueScript II SK(+) as the template as well as the primer pairs shown in .



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